Programmed synthesis of 3D tissues
نویسندگان
چکیده
Cell DNA Gel (all +40U/mL DNase) Media Culture Time CPA Progression Fig 1I A-amine MCF-10A Aprime-lipid n/a n/a n/a 1) 10A&Aprime A-lipid 2) 10A&A 3) 10A&Aprime Fig 2A-C, 3A, S2D-E A-amine MCF-10A Aprime-lipid 9 mg/mL Matrigel 10A Assay Medium 24 hours 1) 10A&Dprime B-amine MCF-10A Bprime-lipid 2) 10A&Bprime D-amine MCF-10A Dprime-lipid 3) 10A&Aprime Fig 2D-H, S2C A-amine MCF-10A Aprime-lipid 9 mg/mL Matrigel 10A Assay Medium 3 hours 1) 10A&Aprime Fig 3A-B A-amine MCF-10A Aprime-lipid 9 mg/mL Matrigel 10A Assay Medium 24 hours 1) 10A&Aprime MCF-10A A-lipid 2) 10A&A Fig 3B-C, S3D A-amine MCF-10A Aprime-lipid 9 mg/mL Matrigel 10A Assay Medium 24 hours 1) 10A&Aprime B-amine MCF-10A A-lipid 2) 10A&(A+B)prime MCF-10A Aprime-lipid mixed with Bprime-lipid 3) 10A&A Fig 3E A-amine MCF-10A Aprime-lipid n/a n/a n/a 1) 10A&Fprime (blue) Famine A-lipid 2) 10A&Aprime (red) Fprime-lipid 3) 10A&F (blue) F-lipid 4) 10A&A (red) 5) 10A&Fprime (blue) 6) 10A&Aprime (red) 7) 10A&F (blue) 8) 10A&A (green) Fig 3F A-amine HMEC-MEP Bprime-lipid 9 mg/mL Matrigel M87A Medium 24 hours 1) MEP&Bprime B-amine HMEC-MEP Aprime-lipid 2) MEP&Aprime HMEC-LEP Aprime-lipid 3) LEP&A HMEC-LEP A-lipid 4) LEP&Aprime 5) LEP&A 6) MEP&Aprime Fig 3G, S3E A-amine MCF-10A Aprime-lipid 6.1 mg/mL Matrigel 10A Assay Medium 24 hours 1) 10A&Aprime MCF-10A A-lipid + 2.1mg/mL collagen I 2) 10A&A 3) 10A&Aprime 4) 10A&A Fig 3H, S3F A-amine MCF-10A Aprime-lipid 6.1 mg/mL Matrigel 10A Assay Medium 24 hours 1) 10A&Bprime B-amine MCF-10A A-lipid + 2.1mg/mL collagen I 2) 10A&Aprime MCF-10A Bprime-lipid 3) 10A&B MCF-10A B-lipid 4) 10A&A 5) 10A&Bprime 6) 10A&Prime Fig 4A-B, S4F A-amine HMEC Aprime-lipid 9 mg/mL Matrigel M87A Medium 24 hours 1) HMEC&Aprime HMEC A-lipid OR 3mg/mL collagen I 2) HMEC&A 3) HMEC&Aprime 4) HMEC&A Fig 4C-E, S4A-D A-amine MCF-10A Aprime-lipid 9 mg/mL Matrigel 10A Assay Medium 80 hours 1) (10A OR 10AT)&Aprime MCF-10A A-lipid 2) (10A OR 10AT)&A MCF-10AT Aprime-lipid MCF-10AT A-lipid Fig 4F-J, S4E-G A-amine MCF-10A Aprime-lipid 9 mg/mL Matrigel 10A Assay Medium 72 hours 1) 10A&Aprime Famine MCF-10A A-lipid 2) 10AT&Fprime MCF-10AT Fprime-lipid 3) 10A&A MCF-10AT F-lipid 4) 10AT&F
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